Dilute immediately before use and put in a clean brown bottle, because dilution also dilutes the decomposition inhibitor. Take care when removing the cap of the reagent bottle, as gas pressure may have built up inside. Solutions at concentrations of 18-28 vol are IRRITANT. Read our standard health & safety guidanceġ Hydrogen peroxide: (See CLEAPSS Hazcard) Solutions less than 18 vol are LOW HAZARD. Collating and comparing class results allows students to look for anomalous and inconsistent data. Groups of three could work to collect results for 5 different concentrations and rotate the roles of apparatus manipulator, result reader and scribe. Individual students may then have time to gather repeat data. You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide. The oxygen produced in 30 seconds is collected over water. This investigation looks at the rate of oxygen production by the catalase in pureed potato as the concentration of hydrogen peroxide varies. Cells make the enzyme catalase to remove hydrogen peroxide. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell. Hydrogen peroxide ( H 2O 2) is a by-product of respiration and is made in all living cells.
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